【摘要】 目的:探讨p97抑制剂NMS-873对食管鳞状细胞癌Eca109细胞增殖、凋亡、迁移的影响。方法:用不同浓度的NMS-873分别处理Eca109细胞24、48、72 h,采用CCK-8法检测细胞增殖;在此基础上选用1、2、3μmol/L NMS-873分别作用于Eca109细胞48 h,采用Annexin V-FITC/PI双染法检测细胞凋亡,Transwell迁移实验检测细胞迁移能力的变化,Ed U荧光法检测细胞增殖。结果:NMS-873可呈时间和剂量依赖性抑制Eca109细胞增殖; 1、2、3μmol/L的NMS-873分别作用于Eca109细胞48 h后,与阴性对照组相比,随着NMS-873浓度的增加,NMS-873组Eca109细胞凋亡率提高,迁移细胞数减少,增殖率降低(P <0. 05)。结论:NMS-873能有效抑制Eca109细胞增殖,促进细胞凋亡,并降低细胞迁移能力。更多还原

【Abstract】 Aim: To investigate the effects of p97 inhibitor NMS-873 on proliferation,apoptosis and migration of esophageal squamous cell carcinoma cell line Eca109. Methods: Eca109 cells were treated with different concentrations of NMS-873 for 24,48,and 72 hours,respectively. CCK-8 assay was used to detect proliferation of Eca109 cells. Based on this,1,2 and 3 μmol/L NMS-873 were chosen and treated Eca109 cells for 48 hours. Annexin V-FITC/PI double staining was used to detect apoptosis,Transwell migration assay was used to detect cell migration ability,and Ed U fluorescence assay was used to detect cell proliferation. Results: CCK-8 results showed that NMS-873 significantly inhibited the proliferation of Eca109 cells in a time-and dose-dependent manner( P < 0. 05). Subsequently,after NMS-873 at 1,2,and 3 μmol/L being used to treat Eca109 cells for 48 hours,compared with the negative control group,with the increase of NMS-873 concentration,the apoptosis rate of Eca109 cells in the NMS-873 groups was significantly increased,while the number of migrated cells and the proliferation rate were significantly decreased( P < 0. 05). Conclusion: NMS-873 can effectively inhibit the proliferation of Eca109 cells,promote cell apoptosis and reduce cell migration capability.更多还原

【分类号】R329.28;R735.1;R730.261;R329.25;R395.9;R971.9;